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Celprogen Inc lung cscs

Gene expression among different cancer types. Notes: Relative gene expression analysis of nine genes that were overexpressed in breast cancer and breast <t>CSCs.</t> The gene expression also was studied in colon and lung cancer, colon CSCs, <t>lung</t> <t>CSCs,</t> embryonic stem cells, and in a reference sample. The experiments were performed in triplicate and a P -value of <0.05 was considered significant. Abbreviations: CSCs, cancer stem cells; ESCs, embryonic stem cells; HP , haptoglobin; SMPD1 , sphingomyelin phosphodiesterase 1, acid lysosomal; SCRIB , scribbled homolog ( Drosophila ); KCMF1 , potassium channel modulatory factor 1; FAM155B , family with sequence similarity 155, member B; PTGER3 , prostaglandin E receptor 3 (subtype EP3); GPR3 , G protein-coupled receptor 3; TMX2 , thioredoxin-related transmembrane protein 2; DDX49 , DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.

Lung Cscs, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lung+cscs/Human+Lung+Cancer+Stem+Cells/pmc04507490-50-12-15
Average 93 stars, based on 7 article reviews

lung cscs - by Bioz Stars, 2026-11

93/100 stars

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1) Product Images from "Identification of genes involved in breast cancer and breast cancer stem cells"

Article Title: Identification of genes involved in breast cancer and breast cancer stem cells

Journal: Breast Cancer : Targets and Therapy

doi: 10.2147/BCTT.S85202

Figure Legend Snippet: Gene expression among different cancer types. Notes: Relative gene expression analysis of nine genes that were overexpressed in breast cancer and breast CSCs. The gene expression also was studied in colon and lung cancer, colon CSCs, lung CSCs, embryonic stem cells, and in a reference sample. The experiments were performed in triplicate and a P -value of <0.05 was considered significant. Abbreviations: CSCs, cancer stem cells; ESCs, embryonic stem cells; HP , haptoglobin; SMPD1 , sphingomyelin phosphodiesterase 1, acid lysosomal; SCRIB , scribbled homolog ( Drosophila ); KCMF1 , potassium channel modulatory factor 1; FAM155B , family with sequence similarity 155, member B; PTGER3 , prostaglandin E receptor 3 (subtype EP3); GPR3 , G protein-coupled receptor 3; TMX2 , thioredoxin-related transmembrane protein 2; DDX49 , DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.

Techniques Used: Expressing, Sequencing

Cell Culture:

Article Title: Identification of genes involved in breast cancer and breast cancer stem cells
Article Snippet: .. Human breast CSCs (36102-29P; Celprogen, CA, USA), colon CSCs (36112-39P; Celprogen), and lung CSCs (36107-34P; Celprogen) were cultured in appropriate growth medium provided by the manufacturer (M36102-29E, M36112-39E, M36107-34E; Celprogen) in 25 cm2 flasks (E36102-29-T25, E36112-39-T25, E36107-34-T25; Celprogen). ..

Article Title: Identification of genes involved in breast cancer and breast cancer stem cells
Article Snippet: .. Human breast CSCs (36102-29P; Celprogen, CA, USA), colon CSCs (36112-39P; Celprogen), and lung CSCs (36107-34P; Celprogen) were cultured in appropriate growth medium provided by the manufacturer (M36102-29E, M36112-39E, M36107-34E; Celprogen) in 25 cm 2 flasks (E36102-29-T25, E36112-39-T25, E36107-34-T25; Celprogen). ..

Article Title: Involvement of retrotransposon L1 in stemness and cellular plasticity.
Article Snippet: .. Epithelial-to-mesenchymal transition (EMT) as well as the reverse process, mesenchymal-to-epithelial transition (MET) is important during embryogenesis.. EMT is also involved in cancer invasion and metastasis, and can generate cells with properties similar to those of stem cells.. Retrotransposons can rearrange the genome by inserting DNA in new loci, thus inducing mutations. .. Epithelial-to-mesenchymal transition (EMT) as well as the reverse process, mesenchymal-to-epithelial transition (MET) is important during embryogenesis.. EMT is also involved in cancer invasion and metastasis, and can generate cells with properties similar to those of stem cells.. Retrotransposons can rearrange the genome by inserting DNA in new loci, thus inducing mutations.

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Journal: Scientific Data

Article Title: A single cell RNAseq benchmark experiment embedding “controlled” cancer heterogeneity

doi: 10.1038/s41597-024-03002-y

Figure Lengend Snippet: Cellranger statistics.

Article Snippet: PC9 (CSC-C4619J) human lung cancer cell line was purchased from Creative Bioarray; DV90 (ACC 307) and HCC78 (ACC 563) human lung cancer cell lines were provided from DSMZ Leibniz Institute.

Techniques:

Quality control of the cells. Low quality cells are characterised by having a low number of genes depicted as present, associated with low ribosomal content and high mitochondrial content. ( A ) A549, ( B ) CCL-185-IG, ( C ) CRL5868, ( D ) DV90, ( E ) HCC78, ( F ) HTB178, ( G ) PC9.

Journal: Scientific Data

Article Title: A single cell RNAseq benchmark experiment embedding “controlled” cancer heterogeneity

doi: 10.1038/s41597-024-03002-y

Figure Lengend Snippet: Quality control of the cells. Low quality cells are characterised by having a low number of genes depicted as present, associated with low ribosomal content and high mitochondrial content. ( A ) A549, ( B ) CCL-185-IG, ( C ) CRL5868, ( D ) DV90, ( E ) HCC78, ( F ) HTB178, ( G ) PC9.

Techniques: Control

Journal: Breast Cancer : Targets and Therapy

Article Title: Identification of genes involved in breast cancer and breast cancer stem cells

doi: 10.2147/BCTT.S85202

Figure Lengend Snippet: Gene expression among different cancer types. Notes: Relative gene expression analysis of nine genes that were overexpressed in breast cancer and breast CSCs. The gene expression also was studied in colon and lung cancer, colon CSCs, lung CSCs, embryonic stem cells, and in a reference sample. The experiments were performed in triplicate and a P -value of <0.05 was considered significant. Abbreviations: CSCs, cancer stem cells; ESCs, embryonic stem cells; HP , haptoglobin; SMPD1 , sphingomyelin phosphodiesterase 1, acid lysosomal; SCRIB , scribbled homolog ( Drosophila ); KCMF1 , potassium channel modulatory factor 1; FAM155B , family with sequence similarity 155, member B; PTGER3 , prostaglandin E receptor 3 (subtype EP3); GPR3 , G protein-coupled receptor 3; TMX2 , thioredoxin-related transmembrane protein 2; DDX49 , DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.

Article Snippet: Human breast CSCs (36102-29P; Celprogen, CA, USA), colon CSCs (36112-39P; Celprogen), and lung CSCs (36107-34P; Celprogen) were cultured in appropriate growth medium provided by the manufacturer (M36102-29E, M36112-39E, M36107-34E; Celprogen) in 25 cm 2 flasks (E36102-29-T25, E36112-39-T25, E36107-34-T25; Celprogen).

Techniques: Expressing, Sequencing

AlPc–PDT tested on cancer cell lines and other PSs evaluated on CSCs for their photodynamic effects.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: AlPc–PDT tested on cancer cell lines and other PSs evaluated on CSCs for their photodynamic effects.

Article Snippet: The cell population was enhanced for CD133+, CD56+ and CD44+ lung CSCs by using the CD133/CD56/CD44 Microbead Kit (Miltenyi Biotec, MicroBead Kit, 130-095-194, 130-100-857, 130-050-401) intended for the positive selection of cells expressing the human CD133/CD56 and CD44 antigen.

Techniques: In Situ, Activity Assay, Expressing, In Vivo, In Vitro

Flow cytometry lung CSC characterization. FL protein detection using flow cytometry. ( a ) Lung CSCs positive for CD133 (FITC), ( b ) lung CSCs positive for CD44 (PE) and ( c ) lung CSCs positive for CD56 (Cy5). All positive samples are overlaid with the control to distinguish between the colour shifts.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: Flow cytometry lung CSC characterization. FL protein detection using flow cytometry. ( a ) Lung CSCs positive for CD133 (FITC), ( b ) lung CSCs positive for CD44 (PE) and ( c ) lung CSCs positive for CD56 (Cy5). All positive samples are overlaid with the control to distinguish between the colour shifts.

Techniques: Flow Cytometry, Control

Immuno fluorescent lung CSC characterization. Fluorescent antigenic detection of the surface markers CD133, CD44 and CD56. ( a ) Immunofluorescent staining of the isolated side population of lung CSCs using FITC. ( b ) Cells counterstained with DAPI indicated by blue FL seen in the nuclei. ( c ) Superimposed fluorescent images of the labelled cells.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: Immuno fluorescent lung CSC characterization. Fluorescent antigenic detection of the surface markers CD133, CD44 and CD56. ( a ) Immunofluorescent staining of the isolated side population of lung CSCs using FITC. ( b ) Cells counterstained with DAPI indicated by blue FL seen in the nuclei. ( c ) Superimposed fluorescent images of the labelled cells.

Techniques: Staining, Isolation

Intracellular localization of AlPcS 4 Cl in isolated lung CSCs. ( a ) Nuclei are counterstained with DAPI, ( b ) mitochondria/ lysosomes fluoresces green (FITC), ( c ) AlPcS 4 Cl auto fluoresces red (DsRED) and ( d ) intermediate yellow is seen in the superimposed images where the green and red channels are merged and FL from the mitochondrion/lysosomes and PS are overlapping.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: Intracellular localization of AlPcS 4 Cl in isolated lung CSCs. ( a ) Nuclei are counterstained with DAPI, ( b ) mitochondria/ lysosomes fluoresces green (FITC), ( c ) AlPcS 4 Cl auto fluoresces red (DsRED) and ( d ) intermediate yellow is seen in the superimposed images where the green and red channels are merged and FL from the mitochondrion/lysosomes and PS are overlapping.

Techniques: Isolation

Morphology of isolated lung CSCs post-PDT. Cells receiving ( a ) no treatment, ( b ) irradiation of 10 J cm −2 and ( c ) PS of 20 µM alone represents healthy viable cells with no cytotoxic morphological changes. ( d ) Lung CSCs that received PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation show indications of apoptotic (green) and necrotic (red) cell death.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: Morphology of isolated lung CSCs post-PDT. Cells receiving ( a ) no treatment, ( b ) irradiation of 10 J cm −2 and ( c ) PS of 20 µM alone represents healthy viable cells with no cytotoxic morphological changes. ( d ) Lung CSCs that received PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation show indications of apoptotic (green) and necrotic (red) cell death.

Techniques: Isolation, Irradiation

LDH cytotoxicity of isolated lung CSCs post-PDT. Cytotoxicity was measured as an absorbance value at 490 nm. All test samples were compared with their respective control cells. No statistical significance was seen when exposing the cells to PS or irradiation alone. A statistical significance of p < 0.001 (***) in LDH cytotoxicity was observed when treating the cells with PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation compared with the control, PS and LILI alone.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: LDH cytotoxicity of isolated lung CSCs post-PDT. Cytotoxicity was measured as an absorbance value at 490 nm. All test samples were compared with their respective control cells. No statistical significance was seen when exposing the cells to PS or irradiation alone. A statistical significance of p < 0.001 (***) in LDH cytotoxicity was observed when treating the cells with PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation compared with the control, PS and LILI alone.

Techniques: Isolation, Control, Irradiation

ATP proliferation of isolated lung CSCs post-PDT. ATP was measured as a luminescent value in RLUs. All test samples were compared with their respective control cells. No statistical significance was seen when exposing the cells to PS or irradiation alone. A statistically significant decrease in ATP proliferation of p < 0.001 (***) was observed when treating the cells with PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: ATP proliferation of isolated lung CSCs post-PDT. ATP was measured as a luminescent value in RLUs. All test samples were compared with their respective control cells. No statistical significance was seen when exposing the cells to PS or irradiation alone. A statistically significant decrease in ATP proliferation of p < 0.001 (***) was observed when treating the cells with PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation.

Techniques: Isolation, Control, Irradiation

Trypan blue viability of isolated lung CSCs post-PDT. Trypan blue was used as a dye exclusion assay, where viable cells excluding the dye were recorded as a percentage value. All test samples were compared with their respective control cells. No statistical significance was seen when exposing the cells to PS or irradiation alone. A statistically significant decrease p < 0.01 (**) in viability was seen when treating the cells with PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation, when compared with the respective control samples.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: Trypan blue viability of isolated lung CSCs post-PDT. Trypan blue was used as a dye exclusion assay, where viable cells excluding the dye were recorded as a percentage value. All test samples were compared with their respective control cells. No statistical significance was seen when exposing the cells to PS or irradiation alone. A statistically significant decrease p < 0.01 (**) in viability was seen when treating the cells with PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation, when compared with the respective control samples.

Techniques: Isolation, Exclusion Assay, Control, Irradiation

Mitochondria membrane potential (Δ ψ m) of isolated lung CSCs post-PDT. ( a ) MitoRed was used to stain mitochondria, where intact mitochondria show a bright red FL and damaged organelles show decreased FL. (i) Lung CSC control, (ii) cells receiving irradiation alone, (iii) cells receiving PS alone without photoactivation and (iv) PDT-treated lung CSCs. Fluorescent intensity was recorded, and all experiments were compared with the control. ( b ) No statistical significance was seen when exposing the cells to PS or irradiation alone. A statistically significant decrease p < 0.05 (*) in Δ ψ m was seen when treating the cells with PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation, when compared with the respective control samples.

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: Mitochondria membrane potential (Δ ψ m) of isolated lung CSCs post-PDT. ( a ) MitoRed was used to stain mitochondria, where intact mitochondria show a bright red FL and damaged organelles show decreased FL. (i) Lung CSC control, (ii) cells receiving irradiation alone, (iii) cells receiving PS alone without photoactivation and (iv) PDT-treated lung CSCs. Fluorescent intensity was recorded, and all experiments were compared with the control. ( b ) No statistical significance was seen when exposing the cells to PS or irradiation alone. A statistically significant decrease p < 0.05 (*) in Δ ψ m was seen when treating the cells with PDT using 20 µM AlPcS 4 Cl and 10 J cm −2 irradiation, when compared with the respective control samples.

Techniques: Membrane, Isolation, Staining, Control, Irradiation

Cell death–annexin VPI of isolated lung CSCs post-PDT. Unstained cells were used to establish the cell population ( a ). A positive control sample was used where apoptosis was induced using hydrogen peroxide ( b ). Cells receiving no treatment were used as the control ( c ). Cells receiving PS or irradiation alone had no significant cell death with viabilities of 92.5% and 93.2%, respectively ( d , e ). Cells receiving PDT showed a statistically significant increase p < 0.001 (***) in early apoptosis (21%), late apoptosis (2.6%) and necrosis (9%) ( f ).

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: Cell death–annexin VPI of isolated lung CSCs post-PDT. Unstained cells were used to establish the cell population ( a ). A positive control sample was used where apoptosis was induced using hydrogen peroxide ( b ). Cells receiving no treatment were used as the control ( c ). Cells receiving PS or irradiation alone had no significant cell death with viabilities of 92.5% and 93.2%, respectively ( d , e ). Cells receiving PDT showed a statistically significant increase p < 0.001 (***) in early apoptosis (21%), late apoptosis (2.6%) and necrosis (9%) ( f ).

Techniques: Isolation, Positive Control, Control, Irradiation

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Figure Lengend Snippet: AlPc–PDT tested on cancer cell lines and other PSs evaluated on CSCs for their photodynamic effects.

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: In Situ, Activity Assay, Expressing, In Vivo, In Vitro

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Flow Cytometry, Control

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Staining, Isolation

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Isolation

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Isolation, Irradiation

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Isolation, Control, Irradiation

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Isolation, Control, Irradiation

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Isolation, Exclusion Assay, Control, Irradiation

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Membrane, Isolation, Staining, Control, Irradiation

Journal: Royal Society Open Science

Article Title: Aluminium (III) phthalocyanine chloride tetrasulphonate is an effective photosensitizer for the eradication of lung cancer stem cells

doi: 10.1098/rsos.210148

Article Snippet: AlPcS 4 Cl – AuNP and AlPcS 4 Cl – AuNP – CD133 , lung CSCs (A549, ATCC® CCL-185) , significant CSC toxicity and cell death; CSC eradication , [ ] .

Techniques: Isolation, Positive Control, Control, Irradiation

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lung cscs (Celprogen Inc)

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